Serology is the study of blood serum and other biological materials (e.g. milk), primarily focussed on the detection of antibodies. These detected Antibodies may originate from maternal transfer (e.g. via colostrum or egg yolk), vaccination or natural exposure to pathogens.

Their presence reflects the immune status of an animal, flock or herd and helps determine protection against specific diseases.

In our serology laboratory, we determine whether animals have developed antibodies against specific pathogens. This is primarily done using ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive and reliable technique for antibody detection. However, other serological tests such as Hemagglutination Inhibition (HI) assays or Virus Neutralisation (VN) assays are within our expertise thanks to a close cooperation with the Virology lab at Poulpharm.

Poulpharm offers diagnostic services for poultry, swine, and cattle, and is continuously expanding with new panels such as for exotic and companion animals. To efficiently handle the high volume of samples while ensuring rapid turnaround times and consistently high-quality results, we rely on automated, robotic processes.

Our team of experienced scientists (PhD, MSc, and BSc level) ensures accurate analysis and interpretation. In addition to routine diagnostics and project-based testing, we provide expert guidance to support your clinical and herd health decisions.

Poulpharm’s serology laboratory operates under both GLP and ISO 17025 accreditation. Validated ELISA assays under ISO 17025 include: IBR gE and gB, Aujeszky gE, M. avium subsp. Paratuberculosis and BVD (antibody and antigen).

In ELISA testing, samples are added to plates coated with pathogen specific antigens. If antibodies are present in the sample, they bind to these antigens. After washing away unbound components, a conjugate is added that recognizes and binds to the antibodies. An enzyme bound on the conjugate invokes a colouring change in the followig step. The intensity of this colour change (measured as optical density or abbreviated OD) correlates with the antibody concentration in the sample. Subsequently, these raw data are transformed into titer values and the relevant interpretation. In case of the PGVS (Personal Gumboro Vaccination Service), further formulas are used to calculate the optimal vaccination day depending on the vaccine used (Hot or Intermediate) and type of animal.