{"id":5080,"date":"2026-04-23T08:54:35","date_gmt":"2026-04-23T07:54:35","guid":{"rendered":"https:\/\/poulpharm.vermis.hu\/?p=5080"},"modified":"2026-04-23T08:57:42","modified_gmt":"2026-04-23T07:57:42","slug":"swine-influenza-reinforced-diagnostics-for-a-persistent-virus","status":"publish","type":"post","link":"https:\/\/poulpharm.vermis.hu\/?p=5080","title":{"rendered":"Swine Influenza: Reinforced Diagnostics For a Persistent Virus"},"content":{"rendered":"

Swine Influenza: Reinforced Diagnostics For a Persistent Virus<\/h1><\/h3><\/div>
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Swine Influenza virus (SIV) causes a contagious respiratory disease in pigs, which spreads rapidly within the herd. Main symptoms are fever, coughing, and lethargy. While most pigs recover, it leads to economic losses due to reduced growth, secondary infections, and treatment costs. Commercial vaccines exist but may be less effective due to viral mutations.<\/span><\/h4>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
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At\u00a0Poulpharm<\/strong>, we provide fast, reliable diagnostics for SIV detection and typing, now including the\u00a0Hemagglutination Inhibition (HI) test.<\/u><\/h4><\/h3><\/div>

How does it work?<\/h2><\/h3><\/div>
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The HI test works by measuring the ability of antibodies in a sample to bind Influenza virus, thus inhibiting the agglutination of red blood cells caused by the virus\u2019s hemagglutinin protein.
\nThree main components \u2014 antibodies, influenza virus, and red blood cells (Figure 1) \u2014 are mixed in wells of a microtiter plate.
\nEach row tests different influenza strains against the same set of antibodies (e.g. from animal blood sampling), allowing hemagglutinin subtype identification. Columns represent increasing antibody dilutions, arranged from low to high. (Figure2).<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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If the antibodies present in the sample neutralize the virus, red blood cells settle at the bottom, forming a small red dot.
\nIn contrast, when viral particles are not neutralized by antibodies, they bind to the red blood cells, forming a diffuse network that prevents the cells from settling.
\nThis setup determines the\u00a0antibody titer<\/strong>, or the highest dilution at which antibodies still inhibit the virus, providing information on immune response strength and specificity.<\/p>\n<\/div>

Therefore, with the HI test, we can:<\/span><\/h4>\n<\/div>